Curcumin-loaded albumin submicron particles with potential as a cancer therapy: an in vitro study

• Nittiya Suwannasom,
• Netsai Sriaksorn,
• Chutamas Thepmalee,
• Krissana Khoothiam,
• Ausanai Prapan,
• Hans Bäumler and
• Chonthida Thephinlap

Beilstein J. Nanotechnol. 2023, 14, 1127–1140, doi:10.3762/bjnano.14.93

• . Particle characterization Entrapment efficiency of CUR The entrapment efficiency (EE%) in CUR-HSA-MPs was calculated based on the following equation: where CURt is the total amount (mass) of CUR in the original incubation solution and CURf is the unloaded CUR amount measured in the supernatant after

Recognition mechanisms of hemoglobin particles by monocytes – CD163 may just be one

• Jonathan-Gabriel Nimz,
• Pichayut Rerkshanandana,
• Chiraphat Kloypan,
• Ulrich Kalus,
• Saranya Chaiwaree,
• Axel Pruß,
• Radostina Georgieva,
• Yu Xiong and
• Hans Bäumler

Beilstein J. Nanotechnol. 2023, 14, 1028–1040, doi:10.3762/bjnano.14.85

• a washing solution of 0.9% NaCl containing 0.2% HSA, particles were resuspended in 0.9% NaCl, checked via light microscopy for aggregation, and stored at 4 °C. Particle characterization To ensure that the subsequent experiments would be carried out on particles that also met the quality requirements

• the pre-fed samples were, was prepared to check for an influence of continued active cell metabolism on the MFI. Table 3 gives an overview of these samples. Results Particle characterization A HbMP suspension was initially adjusted to a concentration of 2% (V/V) HbMPs as described. The hemoglobin

Systematic studies into uniform synthetic protein nanoparticles

• Nahal Habibi,
• Ava Mauser,
• Jeffery E. Raymond and
• Joerg Lahann

Beilstein J. Nanotechnol. 2022, 13, 274–283, doi:10.3762/bjnano.13.22

• SPNPs made from blended proteins can serve as a promising drug delivery carrier owing to the ease of production, the composition versatility, and the control over their size, shape and dispersity. Keywords: nanogels; nanomedicine; particle characterization; protein-based biomaterials; Introduction As

Electrokinetic characterization of synthetic protein nanoparticles

• Daniel F. Quevedo,
• Cody J. Lentz,
• Adriana Coll de Peña,
• Yazmin Hernandez,
• Nahal Habibi,
• Rikako Miki,
• Joerg Lahann and
• Blanca H. Lapizco-Encinas

Beilstein J. Nanotechnol. 2020, 11, 1556–1567, doi:10.3762/bjnano.11.138

• combination of electrophoresis (EP), dielectrophoresis (DEP), and EO flow effects [22], allowing for additional parameters to fine-tune a separation process. Particle characterization and manipulation with EK techniques offer the potential for developing novel separation schemes for the sorting and enrichment

Transient coating of γ-Fe₂O₃ nanoparticles with glutamate for its delivery to and removal from brain nerve terminals

• Konstantin Paliienko,
• Artem Pastukhov,
• Michal Babič,
• Daniel Horák,
• Olga Vasylchenko and
• Tatiana Borisova

Beilstein J. Nanotechnol. 2020, 11, 1381–1393, doi:10.3762/bjnano.11.122

• particles was performed as described above and was also finished with 5 min of sonification. The resulting maghemite colloid of was filtered through sterile Millex syringe filters. Particle characterization by transmission electron microscopy Morphology and size distribution of the particles were

Surface characterization of nanoparticles using near-field light scattering

• Eunsoo Yoo,
• Yizhong Liu,
• Chukwuazam A. Nwasike,
• Sebastian R. Freeman,
• Brian C. DiPaolo,
• Bernardo Cordovez and
• Amber L. Doiron

Beilstein J. Nanotechnol. 2018, 9, 1228–1238, doi:10.3762/bjnano.9.114

• to the neutral PEG shielding the SPIO core. The thicker coating layer of PEG-pseudotannin on IPC-SPIOs shielded the charge even more (−26 mV). Particle characterization via nanophotonic force microscopy The NanoTweezerTM system was used to analyze uncoated SPIOs, PEG-SPIOs, and IPC-SPIOs. Each

Involvement of two uptake mechanisms of gold and iron oxide nanoparticles in a co-exposure scenario using mouse macrophages

• Dimitri Vanhecke,
• Dagmar A. Kuhn,
• Dorleta Jimenez de Aberasturi,
• Sandor Balog,
• Ana Milosevic,
• Dominic Urban,
• Diana Peckys,
• Niels de Jonge,
• Wolfgang J. Parak,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2017, 8, 2396–2409, doi:10.3762/bjnano.8.239

• thickness of 50 nm were custom-made by DENSsolutions B.V. (Delft, Netherlands). The chips had outer dimensions of 2.00 × 2.60 × 0.30 mm3 fitting into the wells of a standard 96-well plate. Particle characterization and exposure The details of the NP synthesis, which followed the protocols published in [44

On the pathway of cellular uptake: new insight into the interaction between the cell membrane and very small nanoparticles

• Claudia Messerschmidt,
• Daniel Hofmann,
• Anja Kroeger,
• Katharina Landfester,
• Volker Mailänder and
• Ingo Lieberwirth

Beilstein J. Nanotechnol. 2016, 7, 1296–1311, doi:10.3762/bjnano.7.121

• may be attributed to the fact that for the smallest particles the uptake mode flips from passive intrusion to protein mediated endocytosis. Materials and Methods Amorphous silica nanoparticles and particle characterization The study was performed by using uncoated, amorphous silica nanoparticles in

Silica-coated upconversion lanthanide nanoparticles: The effect of crystal design on morphology, structure and optical properties

• Uliana Kostiv,
• Miroslav Šlouf,
• Hana Macková,
• Alexander Zhigunov,
• Hana Engstová,
• Katarína Smolková,
• Petr Ježek and
• Daniel Horák

Beilstein J. Nanotechnol. 2015, 6, 2290–2299, doi:10.3762/bjnano.6.235

• functionalization and biocompatibility [27]. We herein report the preparation of OM–NaYF4:Yb3+/Er3+ nanoparticles with controlled morphology, size, composition, phase and luminescence. Thorough particle characterization was performed to elucidate the relationship between synthetic conditions and particle structure

Mammalian cell growth on gold nanoparticle-decorated substrates is influenced by the nanoparticle coating

• Christina Rosman,
• Sebastien Pierrat,
• Marco Tarantola,
• David Schneider,
• Eva Sunnick,
• Andreas Janshoff and
• Carsten Sönnichsen

Beilstein J. Nanotechnol. 2014, 5, 2479–2488, doi:10.3762/bjnano.5.257

• solution consisting of HAuCl4 (75 µL, 0.1 M), CTAB (10 mL, 0.1 M), silver nitrate (AgNO3, 7 µL, 0.04 M), and ascorbic acid (AA, 105 µL, 0.0788 M). Shortly before the experiment, the nanoparticles were washed in two centrifugation steps with water to remove unbound CTAB. Particle characterization Particles

Proinflammatory and cytotoxic response to nanoparticles in precision-cut lung slices

• Stephanie Hirn,
• Nadine Haberl,
• Kateryna Loza,
• Matthias Epple,
• Wolfgang G. Kreyling,
• Barbara Rothen-Rutishauser,
• Markus Rehberg and
• Fritz Krombach

Beilstein J. Nanotechnol. 2014, 5, 2440–2449, doi:10.3762/bjnano.5.253

• vitro as well as in vivo assays. Results Particle characterization The mean hydrodynamic diameter of the Ag-NPs as determined by dynamic light scattering (DLS) was 116 nm and the polydispersity index (PDI) was below 0.3 for all measurements. The mean diameter of the metallic core as determined by

Effects of surface functionalization on the adsorption of human serum albumin onto nanoparticles – a fluorescence correlation spectroscopy study

• Pauline Maffre,
• Stefan Brandholt,
• Karin Nienhaus,
• Li Shang,
• Wolfgang J. Parak and
• G. Ulrich Nienhaus

Beilstein J. Nanotechnol. 2014, 5, 2036–2047, doi:10.3762/bjnano.5.212

• step was repeated to remove residual organic solvent. Polymer-coated QDs with carboxylic acid surface functionalization (Qdot ITK 585) were purchased from Invitrogen (Carlsbad, CA, USA). Particle characterization Optical properties Absorption spectra were measured on a Cary 100 spectrophotometer

Different endocytotic uptake mechanisms for nanoparticles in epithelial cells and macrophages

• Dagmar A. Kuhn,
• Dimitri Vanhecke,
• Benjamin Michen,
• Fabian Blank,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1625–1636, doi:10.3762/bjnano.5.174

• included to demonstrate that not only the applied particle dimensions and uptake pathways are determinant, but the actual cell types as well. Results Particle characterization In the first step, both particles were thoroughly investigated prior to cellular experiments. The particle size measured with

• were grown to confluence for 7 days prior to exposure experiments and J774A.1 cells for 1 day and allowed to adhere prior to use. Particle characterization and exposure The commercially available carboxylate Fluoresbrite™ plain yellow green (cataloged as 1 µm hydrodynamic diameter) and red (cataloged

The protein corona protects against size- and dose-dependent toxicity of amorphous silica nanoparticles

• Dominic Docter,
• Christoph Bantz,
• Dana Westmeier,
• Hajo J. Galla,
• Qiangbin Wang,
• James C. Kirkpatrick,
• Peter Nielsen,
• Michael Maskos and
• Roland H. Stauber

Beilstein J. Nanotechnol. 2014, 5, 1380–1392, doi:10.3762/bjnano.5.151

• potential cytotoxic effects of amorphous silica NP, differing in diameter but not in their negative surface charge. In contrast to other studies, we first performed a state-of-the-art particle characterization. The knowledge of the physico-chemical ‘bar code’ of the NP is an absolute prerequisite in order

Cytotoxic and proinflammatory effects of PVP-coated silver nanoparticles after intratracheal instillation in rats

• Nadine Haberl,
• Stephanie Hirn,
• Alexander Wenk,
• Jörg Diendorf,
• Matthias Epple,
• Blair D. Johnston,
• Fritz Krombach,
• Wolfgang G. Kreyling and
• Carsten Schleh

Beilstein J. Nanotechnol. 2013, 4, 933–940, doi:10.3762/bjnano.4.105

• intratracheally into healthy rats, and cytotoxic and proinflammatory effects were determined by measuring lactate dehydrogenase (LDH), protein, and cytokine levels as well as total and differential cell counts in bronchoalveolar lavage fluid (BALF). Results Particle characterization The mean diameter of the PVP